A comparative study of xylanase producing wild and mutated strains of Streptomyces variabilis VITMUVB02 isolated from Kanyakumari salt pan

The current study aimed to isolate xylanase-producing marine actinobacteria, which is a novel idea; ascribed the challenges associated with culturing of microorganisms. A total 5 actinobacterial colonies were isolated from Kanyakumari sediments. Among them only one isolate, A1 indicated elevated xylanase activity manifesting zone hydrolysis around 20 mm during primary screening. On other hand, secondary screening i.e., sugar estimation by 3,5-dinitrosalicylic acid method, resulted in exhibiting 400 µg/ml enzyme activity. extracted also contained protein concentration 600 µg/ml. Physical and chemical mutation studies carried out over-express production. Chemical involving use EDTA did not show significant increase production thus, was subjected further analyses. showed 28 physical named A'1.75. This 8 more than wild-type strain after UV-exposure for 75 min. A'1.75 exhibited an content 800 potential identified as Streptomyces variabilis VITMUVB02 using 16S rRNA molecular sequencing. weight purified found be 25 kDa kDa, respectively. Fourier Transform Infrared spectroscopy (FT-IR) High-Performance Liquid Chromatography (HPLC) analyses mutagenic effect change spectral functional groups.